The PMI VectorLink manuscript “susceptibility testing of Anopheles malaria vectors with the neonicotinoid insecticide clothianidin; results from 16 African countries, in preparation for indoor residual spraying with new insecticide formulations” was recently published in the peer-reviewed journal, Malaria Journal on August 1, 2019.
While the World Health Organization (WHO) continues to develop a suitable bottle bioassay procedure, there is currently no published guidance regarding clothianidin susceptibility procedures or diagnostic concentrations. The PMI VectorLink Project, therefore, developed a protocol for impregnating filter papers with 2% w/v SumiShield™ 50WG dissolved in distilled water. Susceptibility tests were conducted using insectary-reared reference Anopheles and wild-collected malaria vector species. All tests were conducted within 24 hours of treating papers, with mortality recorded daily for 7 days, due to the slow-acting nature of clothianidin against mosquitoes. Anopheles gambiae sensu lato (s.l.) adults from wild-collected larvae were tested in 14 countries, with wild-collected Anopheles funestus s.l. tested in Mozambique and Zambia.
Read more about the study in the publication here.
For more information, contact:
PMI VectorLink Project, Abt Associates, 6130 Executive Blvd, Rockville, MD 20852, USA